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It was showed that most members contained the motif 2 in the clade 2, clade 4, clade 8, clade 9, clade 22 and clade 23, however, the motif 1 was only present in some NtbHLH members, such as NtbHLH13, NtbHLH14, and NtbHLH20 from the clade 2, NtbHLH38 from the clade 4, NtbHLH84 and NtbHLH95 from the clade 8, NtbHLH107 from the clade 9, NtbHLH290 and NtbHLH292 to NtbHLH295 from the clade 22 and NtbHLH309 from the clade 23, respectively (Fig

However, it is where there is the greatest risk for a positive result